Cytoskeletal research has undergone rapid progress in recent years and is currently an area of research activity. This book provides a wealth of methods and expert advice for investigating the cytoskeletal elements of eukaryotic cells. It focuses on the dynamic and functional aspects of the three major systems of the cytoskeleton--microfilaments, intermediate filaments and microtubules, and their interactions with their associate proteins. Detailed experimental protocols for state-of-the-art techniques are given throughout, and emphasis is placed on recent advances in areas such as video microscopy, molecular biology, immunological analysis, and microchemistry of proteins. This comprehensive volume will be useful to both experienced and novice researchers who wish to apply the technologies available for the study of these important components of eukaryotic cell structure.