Kieleczawa's second volume, DNA Sequencing II: Optimizing the Preparation and Clean-Up, is devoted to the various methods used for extraction, clean-up, quantification, and analysis of DNA. This volume is divided into four comprehensive sections - DNA Purification, Clean-up of DNA Fragments, Storage of DNA, and Quantifying DNA and RNA - and offers the reader an in-depth presentation of DNA technologies. The text also touches upon the many tools and software programs that are found in a typical modern biology laboratory. This fascinating text is a wonderful addition to your molecular biology library. Table of Contents Foreword by Richard Gibbs 1. Preparation of Difficult DNA Templates using Seven Different Commercial Methods 2. Isolation and Use of Bacterial and P1 Bacteriophage-Derived Artificial Chromosomes 3. Preparation of Genomic DNA from Animal Tissue 4. Extraction of Genomic DNA from Plant Tissue 5. Preparation of cDNA Probes for DNA Microarrays 6. Isolation of Nucleic acids from Paraffin-Embedded Archival Tissues and other "Difficult" Sources 7. Comparison of Sequencing Data for DNAs Prepared Using TempliPhi Technology and Traditional Preparation Protocols 8. Whole Genome Amplification using the Multiple Displacement Amplification Reaction 9. Solid Phase Nucleic Acid Purification using Magnetic Beads 10. The Sanger Institute High Through-put Sequencing Pipeline 11. Comparison of PCR Product Clean-up Methods for High Through-put Capillary Electrophores