Substantial advances have been made in the development of protocols enabling organogenesis and whole plant regeneration in cell, organs and tissues in vitro of a large number of plant species, even in some that are considered recalcitrant. Now plant tissue culture is recognized as subject of theoretical and practical importance and has become an integral component of agriculture biotechnology.This fully-updated edition is a comprehensive textbook that provides insights into the major technological advancements on basic techniques, clonal propagation, and haploid and triploid production since the previous edition was published in 2003.